Electrophoretic study of the "easily soluble" proteins of normal and atrophied rabbits' muscles and of normal human muscles.

The compos i t ion of t h e m i x t u r e of t h e "eas i ly so luble" p ro te ins of var ious r abb i t s ' musc les and of h u m a n skele ta l m u s c l e s v i z , t he p ro te ins ex t r ac t ed by KCl -phospha t e of ionic s t r e n g t h o.1B a n d p H 7 . x 5 w a s de t e rmi ned b y e lec t rophores is as descr ibed b y BoscH x. The e x p e r i m e n t s on rabb i t s were carr ied ou t wi th t he v a s t u s la tera l is and t he gas t rocnemius . The differences be tween two n o r m a l con t ra la te ra l musc les were a lways scarce ly perceptible, even w h e n t h e y were r emoved w i t h a week in te rva l . F i f t een r abb i t s were sub jec ted to different t r e a t m e n t s caus ing m u s c u l a r a trophy . As a cont ro l t he n o r m a l cont ra la te ra l musc le was a lways used. All a t roph ica l musc les inves t iga t ed showed t he s ame e lec t rophore t ic pa t t e rn , i n d e p e n d e n t of t he cause of a t rophy , b u t differing ve ry m u c h f rom t he n o r m a l pa t t e rn . I n accordance wi th CREPAX 2 we found t h a t in a t rophy , caused b y dissect ion of t he motor ic nerve, t he pe rcen tage of c o m p o n e n t I I (called m y o a l b u m i n by t he DuBui s soN school) had increased, while t h e percen tages of c o m p o n e n t s VII , V I I I and I X had decreased (percentages of t h e to ta l surface of t he d iagram) . I t f u r t he r appea red t h a t also t h e pe rcen tage of t he level pa r t of t he d i a g r a m be tween c o m p o n e n t s I I a n d V h a d d i s t i nc t l y increased. Simi lar resu l t s were ob ta ined af te r v i t a m i n E defic ien t feeding, s t a rva t i on a n d immobi l i sa t ion . I n d e p e n d e n t of t he cause of t he a t r o p h y an a t roph ied musc le wh ich h a d lost a b o u t ha l f i t s or iginal weight con ta ined per g a b o u t twice t he or iginal a m o u n t of p ro te in I I and of t he p ro te ins ind ica ted b y t he level pa r t of the d iagram, while